Title:

Novel Plasmid DNA Macrocyclic Bottlebrush Polymer

Advances have been made toward generating bottlebrush polymers with unique topologies and assemblies, but synthetic methods and dispersity limitations remain. In this work, biologically derived linear pUC19 plasmid DNA (pDNA) serves as a backbone for synthetically made poly(ethylene glycol) methyl ether mustargen (mPEG-CEA) to alkylate under biologically relevant conditions. By varying the molecular weight mPEG-CEA and the concentration relative to the pDNA, a variety of macrocyclic bottlebrush polymers can be generated with this method. Visualization of the formed bottlebrushes was achieved through the combination of atomic force microscopy (AFM) and agarose gel electrophoresis. This method serves as a facile route to achieve PEGylated DNA materials which can significantly improve DNA’s ability to be used as a material in the future.

Name

Comment*

Comments are viewable only by submitter

Captcha*