Title:

Investigations of Optical Properties: Sensing Hydrogen Peroxide using Activity-Based Sensors

Reactive oxygen species (ROS) are highly reactive molecules that can oxidize a broad range of biological molecules in an uncontrolled manner, such as hydrogen peroxide and hydroxyl radicals. They have been known to play a key role as signaling molecules in regulated cell death (RCD), which changes the morphology of the cell. RCD has many different forms including apoptosis, which is a type of programmed cell death, and ferroptosis, an iron dependent form of cell death that results in peroxidation of lipids. While methods to study ROS generation have been reported, prior research has been limited in the ability to visualize physical changes resulting from ROS generation. The White-Mathieu Lab aims to create membrane anchored activity-based sensors with a turn on fluorescence response, that can be used to visualize the presence of ROS and changes in cell morphology. To contribute to this work, I proposed studies to validate a hydrogen peroxide specific ABS sensor using an analyte screening assay that I developed during this research project. Characterization occurred fluorescence spectroscopy and showed a 16-fold increase in fluorescence after the addition of hydrogen peroxide.

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