Title:

Affinity for Human Dermal Fibroblast Cells to Adhere to Collagen-infused Cryogel Matrix

Cleft palate is a common birth defect caused by incomplete fusion of the palate before birth. Surgical repair is complex and invasive due to the palate’s intricate shape and limited availability of donor tissue. To help address this, we created a cryogel scaffold injected with a 3D hydrogel composed of collagen gel and human dermal fibroblast cells. The cryogel scaffold itself was created by the Hixon lab, and our work allows for future application of this design. When hydrated, the scaffold expands in volume and pore size, making it ideal for tissue growth as simulation of the fluids of the body. The cylindrical shape also matches the size and geometry of typical cleft palate defect and can withstand the pressure inside the body. Collagen I, a natural part of the extracellular matrix (ECM), improves elasticity and supports tissue movement, which allows for a compatible experimental application. The collagen gel is combined with HDF cells to support extracellular matrix formation to become a 3D gel around the cryogel. This matrix offers a promising alternative for cleft palate repair by providing a strong, flexible, and biocompatible structure that supports healing.

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