Title:

Impact of Reelin on Cilia Dynamics in the Cingulate Cortex

Primary cilia are solitary, microtubule-based structures present on nearly every cell type in the human body, including neurons. Primary cilia are known to modulate embryonic neurodevelopment, but little is understood about their role in postnatal neurodevelopment. Defective formation of primary cilia is implicated in the pathogenesis of developmental disorders, collectively known as ciliopathies. Postnatal neurodevelopment involves cortical lamination, where six cellular layers are formed to establish the neocortex, which covers the majority of the cerebral hemisphere. Reelin, a large extracellular matrix glycoprotein, is critical for neuronal migration during cortical lamination. While Reelin’s role in guiding laminar neuronal organization is well characterized, its role in primary cilia dynamics during postnatal neurodevelopment remains unclear. This work investigates whether Reelin influences cilia length and directionality in the mouse cingulate cortex. Using immunostaining and confocal microscopy, we discovered that deletion of Reelin results in disrupted cortical lamination, elongated cilia, and loss of cilia directionality. Interestingly, Reelin deletion had no impact on non-laminated interneurons, suggesting its role in cilia regulation is distinct from its established functions in neuronal migration.

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