Title:

Development of 3D cell cultures through noncytotoxic native chemical ligation

The goal of this project is to establish a route to 3D cell culture utilizing native chemical ligation (NCL) that does not produce any cytotoxic biproducts. While some groups have established success creating 3D scaffolds crosslinked through native chemical ligation (NCL), here we propose a novel variation of the technique as a nontoxic means of cell encapsulation through a β-thiolactone-fuctionalized polymeric backbone. In doing so, we hope to establish an innocuous and facile method to 3D cell scaffold platforms that can be tuned and manipulated to vary aspects such as degradability and hydrogel formation kinetics.

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