Title:

An analysis of SARS-CoV-2 biomarkers at the Durham and Plymouth WWTP’s

This project was completed in correspondence between the Mouser Lab at the University of New Hampshire, the Durham wastewater treatment plant, and the Plymouth wastewater treatment plant. The aim of this project was to analyze the effects of dilution on N1 and N2 biomarkers in wastewater to improve the ability to monitor COVID-19 infections through wastewater-based epidemiology. A relationship between new cases and the concentration of biomarkers in the wastewater was also be analyzed with hopes of understanding how N1 and N2 biomarkers correspond to COVID-19 cases. Additionally, data from Plymouth State University (PSU) was analyzed and compared to data collected by the Mouser research group at the University of New Hampshire (UNH). After analysis, it was determined that on average, a dilution factor of 5.46 was present in samples taken from the Durham WWTP during the fall semester of 2020. Similarly, an average dilution factor of 5.49 was present in samples taken from the Plymouth WWTP during the fall semester of 2020. This resulted in an average case equivalence relationship of 311copies/100mL = 1 COVID-19 case for UNH and 291copies/100mL = 1 COVID-19 case for PSU.

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