Title:

Exogenous Clickable Phospholipids for Imaging Hydrogen Peroxide

A biosensor reacts with a specific analyte to become fluorescent. This allows for in situ real-time monitoring for analyte presence. Analytes of interest include peroxides which produce reactive oxygen species (ROS) that can cause substantial damage to an organism’s cells. Peroxides are capable of degrading membrane function by attacking individual phospholipids within the bilayer, leading to various forms of cancer and disease. The biosensor does not fluoresce without undergoing chemical modification by the analyte, so, the brightness of the activated sensors may quantify the concentration of analyte by luminous intensity. The present gap within the literature is what the most optimal method for introducing fluorescent biosensors into the cellular environment is. This project explores an external synthetic lipid approach where the biosensor is attached to a diglyceride, similar to native phospholipids, outside of the cellular environment then introduced exogenously. So far, this work has produced a moderately pure, synthetic phospholipid capable of biosensor conjugation. Once the azido-phospholipid purification is complete, the probe will be characterized in live cells, then, companioned with other labeling methods to achieve multicolor imaging with more than one biosensor.

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