Title:
Development of a Clickable Probe for Imaging Hydrogen Peroxide in the Bilayer
Poster
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Abstract
Ferroptosis is a complex mode of cell death that plays a significant role in cellular health. Ferroptosis is triggered when reactive oxygen species (ROS), such as hydrogen peroxide, react with iron in the cell. This reaction generates a hydroxyl radical, an unstable molecule that damages phospholipids. Phospholipids are the building blocks of membranes, which separate cells from their environment and envelope organelles. As such, they are essential for cellular function. However, the mechanisms of ferroptosis, and how membranes are affected by this process, is not fully understood. The goal of this research is to investigate the role of membranes in ferroptosis by using chemical tools to visualize membranes when ferroptosis is initiated. This goal will be accomplished through three main aims. Aim 1 is to synthesize molecules that can be used to anchor a probe to the inside of membranes. Aim 2 is to develop a hydrogen peroxide probe with turn-on fluorescence properties and high analyte specificity. Aim 3 is to integrate these molecules into live cells, initiate ferroptosis, and image the fluorescence produced. This research will provide a fundamental understanding of ferroptosis, as well as optimized syntheses and protocols that can be adapted to a variety of probes and analytes.
Authors
First Name |
Last Name |
Erin
|
McCarthy
|
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Submission Details
Conference URC
Event Interdisciplinary Science and Engineering (ISE)
Department Chemistry (ISE)
Group Chemistry Research
Added April 17, 2025, 8:56 p.m.
Updated April 17, 2025, 8:56 p.m.
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