Title:

Decellularizing Spinach Leaf Scaffolds with Microfluidics to Model Angiogenesis

Organ-on-a-chip devices are an emerging technology for the intended use of moving away from animal models. However, vascular systems are not accurately represented as most devices available now lack the hierarchical structures found in the human body. Our goal is to use the pre-existing vessel structures in spinach leaves to achieve a physiologically relevant in vivo model. To do this, the leaves must be decellularized using different detergents and surfactants such as sodium dodecyl sulfate (SDS), bleach, and Triton X-100. Once decellularized, the leaves can be coated in extracellular matrix proteins to promote cell adhesion, and neonatal human dermal fibroblast cells can be seeded onto the scaffold. The cultured system may then be introduced to a novel designed polydimethylsiloxane (PDMS) microchip for a fully functioning vein-on-a-chip device.

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