Title:

Optimization of Methods to Visualize Hydrogen Peroxide at Cellular Membranes

Ferroptosis is a regulated, iron-dependent form of cell death with numerous chemical inputs, driven by the peroxidation of phospholipids, the building blocks of cellular membranes. Since phospholipids are the primary components of all cellular membranes, their destruction through lipid peroxidation causes the breakdown of membranes and ultimately leads to cell death. In an effort to better understand the fundamental mechanisms of ferroptosis, a major research goal in the White-Mathieu lab is to label cellular membranes with pro-fluorescent molecules that allow us to detect analytes implicated in ferroptotic activity and study changes at membranes throughout the progression of this cell death pathway. The goal of this research project is to synthesize a chemical probe that is capable of tagging cellular membranes and optimize the labeling conditions to ensure that an ABS sensor can specifically detect hydrogen peroxide in live cells and report on membrane structure during ferroptosis.

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